Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method

Sofy Permana, Sri Widyarti, Serafinah Indriyani, Dian Siswanto, Aris Soewondo

Abstract


Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting.  Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before.  The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.


Keywords


Porang, Alergy, ELISA, Ig-E

Full Text:

PDF

References


Prosea (1996), Plant Resources of South-East Asia 9, Plants Yielding Non-seed Carbohydrates, Prosea Foundation, Bogor.

Romli, H.U. (2002), Hutan lestari berkat tanaman porang, http://www.Pikiran-rakyat.com/cetak/0702/22/0607.htm. Tanggal akses 25 September 2007.

Anonim (2001), Tanaman Iles-iles Bernilai Ekspor Tinggi, Suara Merdeka, 22 Nopember 2001.

Brown (2000), Aroids Plants of the Arum Family, Second Edition, Timber Press. Portland.

Fu T-J. (2000), Digestion stability as a criterion for protein allergenecity assessment, Annuals of the New York Academy of Sciences. 964, 99-110.

Genua, J.M. & C.J. Hillson (1985), The Occurance, Type and Location of Calcium Oxalate Crystals in the Leaves of Fourteen Species of Araceae, Annals of Botany vol. 56, 351-361.

Hoyer J.R., Asplin, J.R., and Otvos, L. (2001), Phosphorylated osteopontin peptides suppress crystallization by inhibiting the growth of calcium oxalate crystals, Kidney Int , 60, 77–82.

Albeck, S., Addadi, L., Weiner, S. (1996), Regulation of calcite crystal morphology by intracrystalline acidic proteins and glycoproteins, Connec Tissue Res, 35, 419–424.

Lanzalaco AC, Singh PB, Smesko SA, Nancollas GH, Sufrin G, Binette M, Binette JP. (1988), The influence of urinary macromolecules on calcium oxalate monohydrate crystal growth, J Urol , 13, 190–195.

Teng H.H., Dove P.M., Orme C.A. and De Yoreo J.J. (1998), Thermodynamics of calcite growth: baseline for understanding biomineral formation, Science, 1998 Oct 23;282(5389):724- 727. in: Li, X., Zhang D., Lynch-Holm, V. J., Okita, T. W., and Franceschi, V. R. 2003. Isolation of a Crystal Matrix Protein Associated with Calcium Oxalate Precipitation in Vacuoles of Specialized Cells. Plant Physiology, October 2003, Vol. 133, pp. 549–559.

Webb A.W., Cavaletto J.M., Carpita N.C., Lopez L.E., Arnott H.J. 1995. The intravacuolar organic matrix associated with calcium oxalate crystals in leaves of Vitis. Plant J, 7, 633–648.

Bouropoulos N, Weiner S, Addadi L. (2001), Calcium oxalate crystals in tomato and tobacco plants: morphology and in vitro interactions of crystal-associated macromolecules, Chem Eur J, 7, 1881– 1888.

Li, X., Zhang D., Lynch-Holm, V. J., Okita, T. W., and Franceschi, V. R. (2003), Isolation of a Crystal Matrix Protein Associated with Calcium Oxalate Precipitation in Vacuoles of Specialized Cells, Plant Physiology, October 2003, Vol. 133, pp. 549–559.

Stanton, R. Bioconversion of fruit and vegetable wastes. Botany Department, Faculty of Science, Malaya University, Kuala Lumpur, Malaysia

Mastuti, R., Indriyani, S., Roosdiana, A. (2008), Identifikasi Kandungan Asam Oksalat Terlarut Dan Tak Larut Serta Kuantitas Kristal Kalsium Oksalat Secara Mikroskopis Dalam Umbi Tanaman Porang (Amorphophallus oncophyllus Prain ex Hook.f.) Pada Berbagai Fase Perkembangan, Laporan Research Grant IM-HERE Jurusan Biologi FMIPA UB.

Azrianingsih, R., Wahono, T. dan Ekowati, G. (2008), Varian-varian Porang (Amorphophallus oncophyllus Hook.) yang Ditemukan di Jawa Timur, Laporan Research Grant IM-HERE Jurusan Biologi FMIPA UB.

Grime, J. P. (2002), Plant strategies, vegetation process and ecosystem properties. 2nd edition, John Wiley,Chichester.

Lee, S-K, Y-M Ye, S-H Yoon, B-O Lee, S-H Kim, H-S Park (2006), Evaluation and the sensitization rates and identification of IgE-binding components in wild and genetically modified potatoes in patiens with allergic disorders.Clinical and Molecular Allergy, 4, 10.

Rupa, P., Hamilton, K., Cirinna, M., Wilkie, B.N. (2008), A Neonatal Swine Model of Allergy Induced by the Major Food Allergen Chicken Ovomucoid (Gal d1). Int Arch Allergy Immunol, 146:11-18




DOI: http://dx.doi.org/10.21776/ub.natural-b.2012.001.04.14

Refbacks

  • There are currently no refbacks.