Antimalarial Activity (Plasmodium falciparum 3D7) from Sea Sponge Acanthella sp.

faidzil Ikram, Asep Awaludin Prihanto, Choiriyatun Hanifah, Muhammad Iqbal

Abstract


The objective of the research was to get the bioactive candidates from marine sponge Acanthella sp. for anti multi resistant malaria. The first Research was conducted with isolating the active compounds of Acanthella sp. In vitro antimalarial test against Plasmodium falciparum 3D7 was done with several concentrations with positive control using chloroquinon the quantification of the Scizon inhibition was performed with blood staining. Afterward, the infected erythrocytes per 1000 erythrocytes was calculated. IC50 was assayed with probit analysis. The result indicated that all four active fractions showed inhibition on growth of P. Falciparum with different value on IC50. The best fraction is confirmed by fraction B with IC50 of 0.013 ppm.


Keywords


Antimalarial, Plasmodium falciparum 3D7, Acanthella sp.

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References


Saxena S., Neerja Pant, D.C. Jain and R.S. Bhakuni (2003), Antimalarial Agents from Natural Plant Sources, Current Science, 85(9): 1314-1329.

World malaria report (2011), Geneva, World Health Organization, 2011, http://www.who.int/malaria/world_malaria_report_2011/9789241564403_eng.pdf, Diakses pada tanggal 2 Januari 2012.

Hiswani (2007), Gambaran Penyakit dan Vektor Malaria Indonesia, Fakultas Kesehatan Masyarakat, Universitas Sumatera Utara, Medan.

Suparno (2005), Kajian Bioaktif Spon Laut (Forifera : Demosponiae) Suatu Peluang Alternatif Pemanfaatan Ekosistem Karang dalam Bidang Farmasi Makalah Pribadi Falsafah Sains, Institut Pertanian Bogor.

Rao, K. V., NoerKasanah, Subagus Wahyuono, Babu L. Tekwani, Raymon F. Schinazi,§ and Mark T. Hamann (2004), Three New Manzamine Alkaloids, Jurnal Natural Product., Vol:67, 1314-1318.

Trager, W and Jensen, J.B. (1976), Human malaria parasites in continous culture, Science, 193, 673 - 676.

Desjardins, R. W., C. J. Canfield, J. D. Haynes, and J. D. Chulay (1979), Quantitative assessment of antimalarial activity in vitro by a semiautomated microdilution technique, Antimicrob. Agents Chemother, 16:710-718.

Gessler, M.C., Nkunya, M.H.N., Mwasumbi, LB., Heinrich, M., and Toner, M. (1994), Screening Tanzanaian Medicinal Plants for Antimalarial Activity, Acta. Trop., 55, 65 - 67.

Miyaoka, H., Shimomura, M., Kimura, H., Yamada, Y. (1998), Antimalarial Activity of Kalihinol A and New Relative Diterpenoids from the Okinawan Sponge Acanthella sp., Tetrahedron, 54(44): 13467-13474.

Angerhofer, C.K., Pezzuto, J.M., König, G.M, Wright, A.D, Sticher. O. (1992), Antimalarial activity of sesquiterpenes from the marine sponge Acanthella klethra, J Nat Prod., 55(12):1787-1789.


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