The Antibiofilm Activity of Jatropha curcas L.Latex on Staphylococcus aureus ATCC 25923

Rosmalinda Utami, Siti Rahmatul Aini, Dyke Gita Wirasisya

Abstract


Nosocomial infections worldwide shows high rates. One of the causes of nosocomial infections is Staphylococcus aureus. S. aureus as an infectious pathogen is known to form biofilms that often cause failure of antibiotic therapy. Jatropha is one of the plants used by the community in treating various diseases, including infections. The purpose of this study was to determine the jatropha latex antibiofilm activity on S. aureus by microdilution method with crystal violet staining. The results showed that jatropha latex extract had antibiofilm activity, with biofilm inhibition percentages of 150% at concentrations of 0.625 mg/mL, while percent degradation was 189% at concentrations of 5 mg/mL. Jatropha latex extract had biofilm inhibition activity at extract concentrations of 0.625 mg/mL and biofilm degradation at extract concentration of 5 mg/mL. The result of this study can be used as topical drugs for infectious wounds.


Keywords


latex; Jatropha curcas L.; antibacterial; antibiofilm.

References


Masloman, A.P., Kandou, G.D., dan Tilaar, C.R. (2015) Analisis pelaksanaan pencegahan dan pengendalian infeksi di kamar opreasi RSUD Dr Sam Ratulangi Tondano. Jurnal Ilmu Kesehatan Masyarakat UNSRAT (JIKMU). 5 (3), 238–249.

Lister, J.L. dan Horswill, A.R. (2014) Staphylococcus aureus biofilms: recent developments in biofilm dispersal. Frontiers in Cellular and Infection Microbiology. 4.

Kanematsu, H. dan Barry, D.M., Ed. (2015) Biofilm and Materials Science. Springer International Publishing, Cham.

da Costa Krewer, C., Santos Amanso, E., Veneroni Gouveia, G., de Lima Souza, R., da Costa, M.M., dan Aparecido Mota, R. (2015) Resistance to antimicrobials and biofilm formation in Staphylococcus spp. isolated from bovine mastitis in the Northeast of Brazil. Tropical Animal Health and Production. 47 (3), 511–518.

Sharma, A.K., Gangwar, M., Kumar, D., Nath, G., Kumar Sinha, A.S., dan Tripathi, Y.B. (2016) Phytochemical characterization, antimicrobial activity and reducing potential of seed oil, latex, machine oil and presscake of Jatropha curcas. Avicenna journal of phytomedicine. 6 (4), 366–75.

Wagner, H. dan Bladt, S. (1996) Plant Drug Analysis. Springer Berlin Heidelberg, Berlin, Heidelberg.

Cooper, R., Jenkins, L., dan Rowlands, R. (2011) Inhibition of biofilms through the use of manuka honey. Wounds UK. 7 (1), 24–32.

Quave, C.L., Plano, L.R.W., Pantuso, T., dan Bennett, B.C. (2008) Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. Journal of ethnopharmacology. 118 (3), 418–28.

Agustina, S., Ruslan, dan Wiraningtyas, A. (2016) Skrining fitokimia tanaman obat di Kabupaten Bima. Cakra Kimia. 4 (1), 71–76.

Godstime, O., Felix, E., Augustina, J., dan Christopher, E. (2014) Mechanisms of Antimicrobial Actions of Phytochemicals against Enteric Pathogens – A Review. Journal of Pharmaceutical, Chemical and Biological Sciences. 2 (2), 77–85.

Tong, Z., Ni, L., dan Ling, J. (2014) Antibacterial peptide nisin: a potential role in the inhibition of oral pathogenic bacteria. Peptides. 60 32–40.

Shafiei, M., Abdi Ali, A., Shahcheraghi, F., Saboora, A., dan Akbari Noghabi, K. (2014) Eradication of Pseudomonas aeruginosa Biofilms Using the Combination of n-butanolic Cyclamen coum Extract and Ciprofloxacin. Jundishapur journal of microbiology. 7 (2), e14358.

Le, K.Y., Dastgheyb, S., Ho, T. V, dan Otto, M. (2014) Molecular determinants of staphylococcal biofilm dispersal and structuring. Frontiers in cellular and infection microbiology. 4 167.


Refbacks

  • There are currently no refbacks.