The Effect of Taurine on Superoxide Dismutase Enzyme Activity, Malondialdehyde And Histology In Liver Mice (Mus Musculus) Male-Induced Herbicide Glyphosate

Elfa Verda Puspita, Gregorius Nugroho Susanto, Sumardi Sumardi, Endang Linirin Widiastuti


Glyphosate herbicide widely used by farmers in Indonesia. As a result of exposure to herbicides, the production of free radicals or ROS exceeds the body's defense system called oxidative stress. Therefore, the activity of antioxidant enzymes in the body decline and thus can cause tissue damage and certain organ dysfunction. ROS can be suppressed by administration of antioxidants. One source of the antioxidant is taurine. Taurine has a protective effect against oxidation and free radical capture in a variety of cells and tissues against toxic oxidant component. This study aims to determine the effect of taurine in the liver of mice induced herbicide glyphosate on the activity of the enzyme superoxide dismutase (SOD), malondialdehida (MDA), changes in morphology and structure of liver histology. The research used completely randomized design (CRD). Mice were divided into three treatment groups such as, control group (K0), a group of glyphosate (K1) at a dose of 25 mg /kgBW/day orally for 25 days, and a group of taurine+glyphosate (K2) at a dose of taurine 7800 mg/kgBW/day + glyphosate dose 25 mg/kgBW/day orally for 25 days. Each treatment consisted of 8 mice as replication. Results of analysis with one ways ANOVA (P<0.05) followed by the Tukey test showed taurine dose of 7800 mg/kgBW/day can reduce liver cells of male mice that were treated by the herbicide glyphosate but it not be able to increase the levels of SOD and to decrease MDA levels.


glyphosate; liver hystology; malondialdehyde; superoxide dismutase; taurine

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Jasper, R., Locatelli, G.O., Pilati, C., Locatelli, C. (2012), Evaluation of biochemical, hematological and oxidative parameters in mice exposed to the herbicide glyphosate-roundup, Interdiscip Toxicol., 5(3): 133-140.

Djau, R.A. (2009), Faktor Risiko Kejadian Anemia dan Keracunan Pestisida pada pekerja Penyemprot Gulma di Kebun Kelapa Sawit PT. Agro Indomas Kab. Seruyan Kalimantan Tengah, Tesis, Universitas Diponegoro, Semarang.

Harish, R.S., dan Murugan, K. (2011), Oxidative stress indices in natural populations of avicennia alba blume as biomarker of environmental pollution, Environ. Res. 11(8): 1070-1073.

Banks, D. dan Soliman, M.R. (1997), Protective effects of antioxidants against benomyl-induced lipid peroxidation and glutathion depletion in rats, Toxicology, 116: 177-181.

Ning, M., Sasoh, M., Kawanishi, S., Sugiura, H., Piao, F. (2010), Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic, Journal of Biomedical Science, 17 (Suppl 1): S7.

Ozden, S., Catalgol, B., Oktayoglu, S.G., Karatug, A., Bolkent, S., Alpertunaga, B. (2012), Acute effects of methiocarb on oxidative damage and the protective effects of vitamin e and taurine in the liver and kidney of wistar rats, Toxicology and Industrial Health 29(1): 60-71.

Arief, Sjamsul. (2008), Radikal Bebas, Artikel, SMF Ilmu Kesehatan Anak FK Unair/RSU Dr. Soetomo, Surabaya.

Arsana, I.N. (2014), Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) dan Pelatihan Fisik Menurunkan Stres Oksidatif pada Tikus Wistar (Rattus norvegicus) Selama Aktivitas Fisik Maksimal, Disertasi, Universitas Udayana, Denpasar.

Suarsana, I.N., T. Wresdiyati dan A. Suprayogi (2013), Respon stres oksidatif dan pemberian isoflavon terhadap aktivitas enzim superoksida dismutase dan peroksidasi lipid pada hati tikus, J. Ilmu Ternak dan Veteriner, 18(2): 146-152.

Abbasoglu, S. D., Kanbagli, O., Balkan, J., Cevikbas, U., Toker, G.A., dan M. Uysal (2001), The protective effect of taurine against thioacetamide hepatotoxicity of rats, Human and Experimental Toxicology, 20: 23-27.

Hagar, H.H. (2004), The protective effects of taurine against cyclosporine a-induced oxidative stress and hepatotoxicity in rats, Toxicology Letters, 151(2): 335-343.

Tabassum, H., S. Parvez, H. Rehman, B. Banerjee, D. Siemen, dan S. Raisuddin. (2007). Nephrotoxicity and its prevention by taurine in tamoxifen induced oxidative stress in mice. Human & Experimental Toxicology. 26: 509–518.

Akay, C., Yaman, H., Oztosun, M., Cakir, E., Yildirim, A.O., Eyi, Y.E., Agilli, M., Akgul, E.O., Aydin, I., Kaldirim, U., Tuncer, S.K., Eken, A., Oztas, E., Poyrazoglu, Y., Yasar, M., and Ozkan, Y. (2013), The protective effects of taurine on experimental acute pancreatitis in a rat model, Human and Experimental Toxicology, 32(5) : 522-529.

Maulida, A., S. Ilyas dan S. Hutahaean (2013), Pengaruh pemberian vitamin C dan E terhadap gambaran histologis hepar mencit (Mus musculus L.) yang dipajankan Monosodium Glutamat (MSG), Saintia Biologi, 1(2): 1-6.

Yousef, M.I., M.H. Salem, H.Z. Ibrahim, S. Helmi, M.A. Seehy, K. Bertheussen (1995), Toxic Effects of carbofuran and Glyphosate on Semen Characteristics in Rabbit, Environ. Sci. Health. 30: 523-534.

Dallegrave, E., Mantese, F.D., Coelho, R.S., Pereira, J.D., Dalsenter, P.R., Langeloh, A. (2003), The Teratogenic Potential of The Herbicide Glyphosate-Roundup in Wistar Rats. Toxicol Lett. 142(1-2): 45-52.

Contardo-Jara, V., Klingelmann, E., Wiegand, C. (2009), Bioaccumulation of Glyphosate and its Formulation Roundup Ultra in Lumbriculus variegatus and its Effect on Biotrasformation and Antixidant Enzymes, Environ. Pollut., 157: 57-63.

Chahoud I., Ligensa, A., Dietzel, L., Faqi, A.S. (1999), Correlation Between Maternal Toxicity and Embryo Fetal Effect, Reproduction Toxicol. 13(5): 375-381.

Parvez, S., H. Tabassum, B. Banerjee, S. Raisuddin (2008), Taurine Prevents Tamoxifen-Induced Mitochondrial Oxidative Damage in Mice, Basic & Clinical Pharmacology & Toxicology. 102: 382-387.

Chen, S.W., Chen, Y.X., Shi, J., Lin, Y., Xie, W.F. (2006), The Restorative Effect of Taurine on Experimental Nonalcoholic Steatohepatitis, Dig Dis Sci. 51: 2225-2234.

Chen, X., Sebastian, B.M., Tang, H., McMullen, M., Axhemi, A., Jacobsen, D., Nagy, L. (2009), Taurine Supplementation Prevents Ethanol-Induced Decrease in Serum Adinopectin and Reduce Hepatic Steatosis in Rats, Hepatology. 49(5): 1554-1562.

Du, H., You, J., Xu, Z., Park, J., Kim, S., Chang, K. (2010), Antiobesity and Hypolipidemic Effects of Lotus Leaf Hot Water Extract with Taurine Supplementation in Rats Fed A High Fat Diet, Biomedical Science, 17(1): S42.

Son, T.G., S. Camandola, M.P. Mattson (2008), Hormetic Dietary Phytochemicals, Neuromol Med,. 10: 236-246.

Chen Y.X., Wang, S.C., Zhao, G.N. (1992), Effect of Taurine on Calcium Uptake and Release by Isolated Rat Liver Mitochondria, Fourth Milid Med Univ., 13: 282-283.

Kozumbo, W.J., Agarwal, S., Koren, H.S. (1992), Breakage and Binding of DNA by Reaction Product of Hypochlorous Acid with Aniline, 1-Naphthylamine or 1-Naphthol. Toxicol Appl Pharmacol, 115: 107-115.

Redmond, H.P., Wang, J.H., Bouchier-Hayes, D. (1996), Taurine Attenuates Nitric Oxide and Reactive Oxygen Intermediate-Dependent Hepatocyte Injury, Arch Surg. 131: 1280-1288.

Schaffer, S., Azuma, J., Takahashi, K., Mozaffari, M. (2003), Why is Taurine Cytoprotective?, Adv Exp Med Biol., 526: 307-321.

Marciniak, A., Brzeszczynska, J., Gwozdzinski, K., dan Jegier, A. (2009), Antioxidant Capacity and Physical Exercise. Biology of Sport, 26(3): 197-213.

Caille, C., Heu, C., Guyon, C. and Nicod, L. (2010), Morphological Damages of a Glyphosate-treated Human Keratinocyte Cell Line Revealed by A Micro-to Nanoscale Miscroscopik Investigation, Cell Biol Toxicol, 26: 331-339.

El-Sayed, W.M., Alkahtani, M., Abdel-Monem, A.M. (2011), Prophylactic and Therapeutic Effects of Taurine Against aluminium-Induced Acute Hepatotoxicity in Mice, Hazardous Materials. 192: 880-886.



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