Effect of Hydroxyquinoline on Preparation of Chromosome Roots and Garlic Garlic (Allium sativum L.)

Arbaul Fauziah


1The effective method in making chromosome prepararation was able to count the number of chromosome easier, especially for analysis the ploidy level in plants. Garlic (Allium sativum L.) is one of the plants that the chromosome is difficult to be counted because their chromosomes are long and difficult to spread during the squashing. This research was to find out the effect of hydroxiquinoline on making chromosome preparation of root and callus in garlic. The steps of making chromosome preparation were fixation in Farmer’s fluid, put in hydroxiquinoline for an hour, hydrolysis in 1N HCl for two minutes at 60°C, put in Carnoy for 30 minutes, and stained in 1% aceto orcein for 20 minutes. The treatment were gift hydroxiquinoline for an hour after put in fixative fluid and the control were making preparation without gift hydroxiquinoline. The result of this research suggest that the method of making preparation by treatment with hydroxiquinoline for an hour after fixation produced the chromosomes that the stain were sharp and contrast, and also it produced well spread chromosomes that were clearly defined of both callus and root tip in garlic.



Allium sativum L.; Hydroxiquinoline; callus; chromosome; root

Full Text:



Owen, H. R. & A. Raymond Miller (1993), A Comparison of Staining Techniques for Somatic Chromosomes of Strawberry, Hortscience 28 (2):155-156.

Mirzaghaderi, G. (2010), A Simple Metaphase Chromosome Preparation from Meristematic Root Tip Cells of Wheat for Karyotyping or In Situ Hybridization, African Journal of Biotechnology, 9 (3): 314-318.

Tjio, J. H. & Levan, A. (1950), The Use of Oxyquinoline in Chromosome Analysis, - Anales Est. Exp. Aula Dei (2): 2144.

Ahloowalia, B. S. (1965), A Root Tip Squash Technique for Screening Chromosome Number in Lolium. Euphytica (14): 170-172.

Winarto, B. (2011), Pewarnaan Kromosom dan Pemanfaatannya dalam Penentuan Tingkat Ploidi Eksplan Hasil Kultur Anter Anthurium. J. Hort 21 (2): 113-123.

Nishibayashi, S., Hayashi, Y., Kyozuka, J. & Shimamoto K. (1989). Chromosome Variations in Protoplast Derived Calli and in Plant Regenerated from the Calli of Cultivated Rice (Oryza sativa L.). Jpn. J. Genet, 64: 355-361.

Yamasiro, T., Kazuo, S. & Masayuki, M. (2005), Chromosome Numbers of Isodon (Lamiaceae) in Japan. Acta Phytotax, Geobot. 56 (3):241-246

Araki, H., H. Shimazaki, Y. Hirata, T. Oridate, T. Harada, and T. Yakuwa (1992), Chromosome Number Variation of Callus Cells and Regenerated Plants in Asparagus officinalis L. Plant Tissue Culture Letters 9 (3): 169-175),

Fluminhan, A., M.L.R.D. Perecin, and J.A. Dos Santos, (1996), Evidence for Heterochromatin Involvement in Chromosome Breakage in Maize Callus Culture.Annals of Botany 78: 73-81.

Anamthawat, J. K. (2003), Preparation of Chromosomes from Plant Leaf Meristems for Karyotype Analysis and In Situ Hybridization, Methods Cell Sci, 21: 91-95

DOI: http://dx.doi.org/10.21776/ub.natural-b.2015.003.01.9


  • There are currently no refbacks.