The Effect of Exposure to Gamma Radiation And Giving Extracts Of White Watermelon (Citrullus vulgaris Schrad) Part of Kidney Health In Animal Trying Mice

Pujianti B. Donuata, Muhaimin Rifa'i, Unggul P. Juswono

Abstract


Cancer is a worldwide problem. People take medication in late stadium therefore radioteraphy plays important role in healing cancer. Side effect of cancer therapy such as cancers of urethra, colon, stomach or rectum may have bad influence for kidney which causes the decrease kidney function. Goal of this research is to understand the influence of gamma radiation on urine and kidney tubules necrosis. Mice assigned into two groups, which are extract and non-extract radiation groups. Each group has five variations of radiation doses, including 200rad, 250rad, 300rad, 350rad, and 400rad. White part extract of watermelon (Citrullus vulgaris Schrad) also used with five variations including 0.3mL, 0.6mL, 0.9mL and 1.2mL. Non-extract radiation group was only exposed to radiation without treatment with white part extract of watermelon. Profile of kidney health can be seen from the percentage of epithelium, erythrocyte and leukocyte after mice were put into metabolism cage for 24hours and percentage of kidney tubules necrosis observed under Microscope BX-51 and analyzed with Ms.Excel. Result of research concludes that gamma radiation increase epithelium, erythrocyte and leukocyte in urine, and kidney tubules necrosis. White part extract of watermelon can improve kidney health by reducing the percentage of epithelium, erythrocyte leukocyte and kidney tubules necrosis of mouse.


Keywords


Gamma Radiation; Citrullus vulgaris Schrad; Kidney Health

Full Text:

PDF

References


Syahrun MH, Suhana N, Sudarmo S, Tjokronogoro A. (1984), Pengaruh Radiasi Terhadap Sistem Sistem Pertahanan Tubeh Seluler Pada Penderita Kanker Nasofarng, MKI.

Santoso, H.B dan Nurliani, A. (2006), Efek Doksisilin Selama Masa Organogenesis pada Struktur Histologi Organ Hati dan Ginjal Fetus Mencit, Bioscientiae 3(1): 15-27.

Susetyarini, Eko (2007), Pengaruh Dekok Daun Beluntas (Pluchea indica Less) Terhadap LD 50 (Toksisitas Akut) tikus putih jantan (Ratus nurwegicus), Laporan Penelitian, Lemlit UMM.

Soekmanto A, (2003), Pengaruh fraksi aktif tumbuhan Aglaia angustifolia terhadap ginjal mencit (Mus musculus), Natur Indonesia 6(1): 49-52

OL Erukainure, OV Oke, AO Daramola, SO Adenekan, EE Umanholen, (2010), Improvement of the biomedical properties of watermelon rinds subjected to Saccharomyces cerevisae solid media fermentation, Pakistan Jurnal of Nutrition 9(88), 806-809.

Sutapa, G. N. (2010), Respon Radiadaptasi Terhadap Kuantitas Sel Darah Putih (Leukosit) Mencit (Mus muculus) dengan Radiasi Gamma Co-60 Secara In-Vivo, Fisika Medis, Skripsi, Universitas Indonesia, Jakarta.

Ok-Jun Lee, Kyu-Rae Kim, Dae Woon Eom, Hyun Jung Kim, Na-Hye Myong, Jae Y. Ro (2004), Radiation-Induced Epithelial Proliferation Mimicking Invasive Carcinoma of the Urinary Bladder-A Report of 2 Cases, Departments of Pathology, University of Ulsan Cheon-An, Korea.

Suryohudoyo, P. (2012), Oksidan, Antioksidan dan Radikal Bebas, Biokimia Kedokteran Unair, Surabaya.

Halliwell, B. (1991), Reactive Oxygen Species in Living System: Source, Biochemistry and Role in Human Diseases. Am. J. Med: suppl. 3C, paper 3C-14S.

Effendi, I. & Markum, H.M.S. (2007), Pemeriksaan Penunjang pada Penyakit Ginjal, Penerbitan Departemen Ilmu Penyakit Dalam FKUI: 9-505.

Suratmo. (2012), Reaksi Radikal Bebas dengan Suatu Materi, Kimia FMIPA Universitas Brawijaya, Malang.

Han, W. & K.N. Yu (2010), Ionizing Radiation, DNA Double Strand Break and Mutation. Advances in Genetics research. 4: 1-13.

Proctor P.H., Reynolds E.S. (1984), Free radicals and disease in man, Physiol Chem Phys Med NMR.

Di Mascio, P., Kaiser, S., Sies, H. (1989). Lycopene as the Most Efficient Biological Carotenoid Singlet Oxygen Quencher, Archives of Biochemistry and Biophysics.

Pratt in Shui, G., Wong, S.P., Leong, L. P. (2004), Characterization of Antioxidants and Change of Antioxidant Levels during Storage of Manilkara zapota L. Agricultural and Food Chemistry, 52. 7834-7841.

Sutapa, G. N. (2010), Respon Radiadaptasi Terhadap Kuantitas Sel Darah Putih (Leukosit) Mencit (Mus muculus) dengan Radiasi Gamma Co-60 Secara In-Vivo. Fisika Medis, Skripsi, Universitas Indonesia. Jakarta.

Halliwell, B. & J. M. C. Gutteridge (1999), Free Radicals in Biology and Medicine, Oxford University Press, New York.

Nguyen M.L., Schwartz S.J., (1999), Lycopene: chemical and biological properties, Food Tech 53:38–45.

Miladiyah, I. (2004), Isoflavon Kedelai Sebagai Alternatif Terapi Sulih Hormon (TSH), Kedokteran Yarsi 12: 94-99.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2014.002.04.15

Refbacks

  • There are currently no refbacks.