Effect of Rosella Petal Extract (Hibiscus sabdariffa L.) On SOD Activity and Mouse Eye Rat (Rattus Wistar) Exposed to Gamma Radiation

Rara Pramita, Chomsin S. Widodo, Unggul P. Juswono

Abstract


Measurement activity of superoxide dismutase (SOD) and Malondhyaldehyde (MDA) to analyze the effect of roselle calyx extract (Hibiscus sabdariffa L) after exposure 300 rad gamma radiation. 35 male white rats (Rattus Wistar) were exposed to fractination dose of 300 rad for 7 day, are given in the sonde 2cc/days roselle extract for 16 days with a dose variation 62.5 mg/200 g BW, 125 mg/200 g BW, 250 mg/200 g BW, 400 mg/200 g BW, and 550 mg/200 g BW. Measurement of the activity of SOD and MDA were done by thiobarbituric acid (TBA) methode. Extract Roselle (Hibiscus sabdariffa L) 250 mg/200 gr BW increase the activity of superoxide dismutase (SOD) and decrease Malondialdehyde (MDA) after being exposed to radiation of 300 rad.


Keywords


Gamma radiation; Roselle Extract; SOD; MDA

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References


Cimen Ozgen, S., D. Dokmeci, M. Akpolat, C. Hakan Karadag, O. Gunduz, H. Erbas, O. Benian, C. Uzal dan F. Nesrin Turan (2012), The Protective Effect of Curcumin on Ionizing Radiation-induced Cataractogenesis in Rats, Balkan Medical Journal, 29(4): 358.

Dragan, A., D.A. Dusanka, B. Drago dan T. Nenad (2002), Structure Radical Scavenging Activity Relationship of Flavonoids. Croatica Chemica Acta, 76(1): 55-61.

ElAttar, T. M. A. (1978), Prostaglandins: physiology, biochemistry, pharmacology and clinical applications. Journal of Oral Pathology & Medicine, 7(5): 253-282.

Giusti, M.M. dan R.E. Wrolstad (2001), Characterization and Measurement of Anthocyanins by UV-Visible Spectroscopy dalam Current Protocols in Food Analytical Chemistry. John Wiley & Sons, Inc. pp.

Jiangmei, W., S. Stefan dan P.V. Algvere (2006), Photochemical Damage of the Retina, Elsevier Survey of Ophthalmology, 51(5).

Joe, A.V. (2006), Oxidative stress in cataracts, Pathophysiology, 13: 151-162.

Kocer, I., S. Taysi, M. Ertekin, I. Karslioglu, A. Gepdiremen, O. Sezen dan K. Serifoglu (2007), The effect of L-carnitine in the prevention of ionizing radiation-induced cataracts: a rat model, Graefe's Archive of Clinical & Experimental Ophthalmology. 245(4): 558.

Shirazi, A., G.H. haddadi, F. Asadi-Amoli, S. Sakhaee, M. Ghazi-Khansari dan A. Avand (2011), Radioprotective Effect of Melatonin in Reducing Oxidative Stress in Rat Lenses, Cell Journal. 13(2): 79-82.

Suwandi, T. (2012), Pengembangan potensi antibakteri kelopak bunga Hibiscus sabdariffa L (Rosela) terhadap Streptococcus sanguinis penginduksi gingivitis menuju obat herbal terstandar, Fakultas Kedokteran Gigi, Universitas Indonesia, Jakarta.

Zulkarnain (2012), Analisis Pengaruh Penyinaran Sinar Gamma terhadap Kadar Insulin Pankreas Sebelum dan Sesudah Permberian Ekstrak Buah Pare (M. charantia) Pada Mencit Yang Dibebani Glukosa, Tesis, Fisika FMIPA, Universitas Brawijaya, Malang.

Zuzana, F., Z. Friedo, B. Ladislav dan R. Jaroslav (2012), Indicators of oxidative stress after ionizing and/or non-ionizing radiation: Superoxide dismutase and malondialdehyde, Journal of Photochemistry and Photobiology. 117: 111-114.




DOI: http://dx.doi.org/10.21776/ub.natural-b.2014.002.04.13

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