Yogurt Supplementation In Rats (Rattus Norvegicus) Foods Affected by Formaldehyde Exposure to Enzymatic Antioxidant Activity at Hepar tissue

Chanif Mahdi, Aulanniam Aulanniam


Yogurt or yogurt is fermented milk products are rich in various vitamins and amino acids, has potential as a potential source of exogenous antioxidants. Formaldehyde is an organic compound or the aldehyde group of the most simple alkanal are toxic and carcinogenic, presenting  in the body can act as a source of compounds reactive oxygen species (ROS) and free radicals that damage cells and tissues are organs. The purpose of this study was to determine the effects of formaldehyde as a toxic compound, and yogurt as detoksikan of antioxidant activity in this regard is the enzymatic antioxidant activity superoksid dismutase (SOD) and levels of reduced glutathione (GSH). The results showed that exposure to various doses of formaldehyde in food rat (Rattus norvegicus) respectively is 0 ppm, 25 ppm 50 ppm 75 ppm and 100 ppm is very real effect (P <0.01) toward decreased antioxidant enzymatic activity in this regard is the reduction of activity superoksid dismutase (SOD) and GSH levels).The results also showed that treatment of yogurt supplementation at 2 ml per cow per day in rats (Rattus norvegicus) were exposed to formaldehyde are not able to change the effect of formaldehyde exposure to the decrease of antioxidant activity (SOD activity and GSH levels) with the significance level remains highly significant (P <0.01)


Yogurt, Enzimatic antioxidant, Oxidative damage, Hepar

Full Text:



Troco, C., Pardo, R., Rafecal,I., Virgile, J., Remesar, X., Fernandes, JA., and Alemany, M. (1998), Formaldehyde dirived form diatery aspartam bind tissue component in vivo. Departement de bioquimca biologia Universitiet dBalcelona. 08028 Barcelona Spain. P 1-4.

Teng, S. Beard, K., Pourahman, J., Mondem, M., Easson, E., Poon, R., Obrean, PJ, The formaldehyde metabolic detoxification enzyme systems and moleculer cytotoxic mechanism in isolated rat hepatocytes, Chem. Boil interact. Vol. 130- 132. p 285-296.

Chen,F., Castronova,V., Shi,X., Demen, LM. (1999), In sight into role of Nuclear Factor –κB, anubiqitos transcription factor in the initation desease, Clinical chemistry 45 : 7-17.

Hancock, JT., Desikin,R., and Neil,SJ. (2001), Biochemical transaction. Biochem. Soc. Trans. 29 : 345- 350.

Lee, WM. (2003), Drug induced hepatotoxicity. N Engl J Med. 349 (5) : 474-484.

Gulec , M ., Gurel, A., and Armerte, F. (2006), Vitamin E protect again oxidative damage caused by formaldehyde in the liver and plasma rat.

Plotken, M. (2007), Detoxification & Drainage . http://www.heelusa.com/file/mice/plotken.detox.october.no.29/pdf.

Kumar, V., Cotarns, RS., Robbin, SL. (2003), Robbins basic pathology. 7rd ed. Arrangement with elsiver Inc. New York USA. p 3-31; 113-150.

Eltean (2005), The nutrition value of yogurt, Eltean incoparated, Sdn.Bhd, Perak Malaysia, http:/www.eltean.com/yoghurt.html.

Bray,TM. (2006), The role of free radical in nutrition and prevention of chronic desease, College of health and human science, Origon,USA. p. 1-37.

Laboratorium Biomedik (2003), Protokol pemeriksaan MDA, SOD, katalase plasma dan jaringan, Fakultas Kedokteran, Universitas Brawijaya, Malang.

Laboratorium Farmakologi (2007), Intro of oxidant-antioxidant measurement, Fakultas Kedokteran Universitas Brawijaya Malang.

BioVision (2005), ApoGSHTM Glutathion colorimetric detection kit. Catalog K 261-100 assays, Biovision reseach product. 980 Linda vista avenoue, Mountain View. CA. 94043 USA

Guven, A., and Kaya, N. (2005), Ditermination of reduced glutathione, glutathione transferase, peroxidase, and s- transferase activity, Application to cesplater- inducer toxicity. Clin Biochem. 23 : 501- 504.

Miller, K. (2002), Immunocytochemical techniques. Theory and practice of histological techniques, Fifth edition, Churchill livingstode. London. p 421- 464.

Halliwell, B., and Gutteridge, JMC. (1999), Free radical in Biology and medicine. Third edition, Oxford, Oxford University Press. p 1-35; 246- 350.

Suryohudoyo, p. (2000), Ilmu Kedokteran Molekuler, CV Sagung Seto Cetakan pertama, pg. 31-47.

Dassagayam, TPA., Tilac,JC., Bolor, KK., Sone, KS., Ghoshodfi,S., and Dile, RD. (2004), Free radical and antioxidant in human health, Current statusand future prospect, Radiation biology and health science division, JAPI Vol 52 : 2-7.

Gasdetection (2007), Formaldehyde human health effect http://www.gasdetection.com/TECH/hcho.html. p 18-25.

DOI: http://dx.doi.org/10.21776/ub.natural-b.2011.001.02.13


  • There are currently no refbacks.